Services
What We Offer
The PCPC provides the highest-quality cutting edge proteomic technology to researchers in a variety of fields such as health, agriculture, forestry, energy, environment and more.
Quantitative Proteomics
Services in Quantitative Proteomics include:
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Targeted Quantitation: LC-MRM/MS allows targeted quantitation for the precise measurement of a large numbers of proteins.
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Protein Enrichment: Using enrichment strategies, such as immune-enrichment, targeted assays can be made more sensitive and specific.
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Untargeted Quantitation: approaches includes label-free quantitation (LFQ), chemical tagging (iTRAQ and TMT) and metabolic labeling (SILAC)
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Tissue Imaging: MALDI Imaging enables the 2D spatial localization of proteins and peptides in tissues
Functional Proteomics
Services in Functional Proteomics include:
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Post-Translational Modification (PTM) Analysis: Detection and quantification of PTMs, including phosphorylation, methylation, acetylation and others in samples of low (purified proteins/protein complexes) and high (cells, tissues, biofluids) complexity.
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Protein Characterization: Accurate mass determination of proteins and protein fragments, as well as detection of mutations and deletions in expressed proteins.
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Protein Interaction Network Mapping: multiple techniques are available to identify protein-protein interactions in vitro and within cells
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Protein Identification: identification and sequencing of unknown proteins, antibodies or protein isoforms
Bioinformatics
Services in Bioinformatics include:
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Software tools: to aid in the development and validation of targeted MRM assays
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Advanced bioinformatics and biostatistics: Analysis and interpretation of MS datasets
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Databases: the PCPC has created a number of data and knowledgebases to support assay development and data interpretation
Structural Proteomics
Services in Structural Proteomics:
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Hydrogen Deuterium Exchange (HDX): HDX can be used to determine the degree of solvent accessibility of regions of a protein, which can inform on secondary and tertiary structure, as well as providing information on protein-protein or protein-ligand complexes.
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Crosslinking: Uses various chemical cross-linking compounds with different functional groups and lengths to inform on spatial interactions within and between proteins