Services

What We Offer

The PCPC provides the highest-quality cutting edge proteomic technology to researchers in a variety of fields such as health, agriculture, forestry, energy, environment and more.

Quantitative Proteomics

Services in Quantitative Proteomics include:

  • Targeted Quantitation: LC-MRM/MS allows targeted quantitation for the precise measurement of a large numbers of proteins.  

  • Protein Enrichment: Using enrichment strategies, such as immune-enrichment, targeted assays can be made more sensitive and specific. 

  • Untargeted Quantitation: approaches includes label-free quantitation (LFQ), chemical tagging (iTRAQ and TMT) and metabolic labeling (SILAC) 

  • Tissue Imaging: MALDI Imaging enables the 2D spatial localization of proteins and peptides in tissues 

Functional Proteomics

Services in Functional Proteomics include: 

  • Post-Translational Modification (PTM) Analysis: Detection and quantification of PTMs, including phosphorylation, methylation, acetylation and others in samples of low (purified proteins/protein complexes) and high (cells, tissues, biofluids) complexity. 

  • Protein Characterization: Accurate mass determination of proteins and protein fragments, as well as detection of mutations and deletions in expressed proteins.  

  • Protein Interaction Network Mapping: multiple techniques are available to identify protein-protein interactions in vitro and within cells  

  • Protein Identification:  identification and sequencing of unknown proteins, antibodies or protein isoforms  

Bioinformatics 

Services in Bioinformatics include: 

  • Software tools: to aid in the development and validation of targeted MRM assays  

  • Advanced bioinformatics and biostatistics: Analysis and interpretation of MS datasets  

  • Databases: the PCPC has created a number of data and knowledgebases to support assay development and data interpretation 

Structural Proteomics 

Services in Structural Proteomics: 

  • Hydrogen Deuterium Exchange (HDX): HDX can be used to determine the degree of solvent accessibility of regions of a protein, which can inform on secondary and tertiary structure, as well as providing information on protein-protein or protein-ligand complexes. 

  • Crosslinking: Uses various chemical cross-linking compounds with different functional groups and lengths to inform on spatial interactions within and between proteins 

Custom Services

Find out more about our customizable service offerings; we work with you to suit your unique research needs

Our Funding Partners

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© Pan-Canadian Proteomic Centre 2019